Reporter

Part:BBa_K3809011:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-07)


mRFP with Linker, His Tag and Signal Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 651
    Illegal AgeI site found at 763
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to optimize the codons for its expression in E. coli.


Source

The Signal Peptide sequence NPS4 was obtained from BBa_K3606042, the linker was a modified version of BBa_K1486004, with the addition of a cistein; we based the sequence of mRFP1 on part BBa_J23100.

References