Reporter
Part:BBa_K3809011:Design
Designed by: Francisco Javier Castañeda Villagran Group: iGEM21_TecCEM (2021-10-07)
mRFP with Linker, His Tag and Signal Peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 651
Illegal AgeI site found at 763 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to optimize the codons for its expression in E. coli.
Source
The Signal Peptide sequence NPS4 was obtained from BBa_K3606042, the linker was a modified version of BBa_K1486004, with the addition of a cistein; we based the sequence of mRFP1 on part BBa_J23100.